pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)
(Plasmid #86874)

• Purpose: To express MORF4L1 (iso2) with a FLAG tag at N-terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pcDNA 5/FR/TO
• Backbone size w/o insert (bp): 5070
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MORF4L1
• Alt name: MRG15
• Species: H. sapiens (human)
• Insert Size (bp): 972
• Mutation: none
• GenBank ID: NM_006791.3
• Entrez Gene: MORF4L1 (a.k.a. Eaf3, FWP006, HsT17725, MEAF3, MORFRG15, MRG15, S863-6)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry