pVENUS-N1-POLR2G-GFP
(Plasmid #86872)

• Purpose: To express POLR2G (RPB7) tagged with VENUS at C terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pVENUS-N1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5216
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POLR2G
• Alt name: RPB7
• Species: H. sapiens (human)
• Insert Size (bp): 516
• Mutation: none
• GenBank ID: NM_002696.2.
• Entrez Gene: POLR2G (a.k.a. RPB19, RPB7, hRPB19, hsRPB7)
• Promoter: CMV
• Tag / Fusion Protein:
  • VENUS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pEGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry