pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG
(Plasmid #86871)

• Purpose: To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SF3B6
• Alt name: Splicing factor 3B subunit 6
• Alt name: SF3B14
• Species: H. sapiens (human)
• Insert Size (bp): 306
• Mutation: amino acids 7-29 deletion
• GenBank ID: NM_016047.3
• Entrez Gene: SF3B6 (a.k.a. CGI-110, HSPC175, Ht006, P14, SAP14, SAP14a, SF3B14, SF3B14a)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pArev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry