pcDNA5/FRT/TO-FNTB-HA
(Plasmid #86869)

• Purpose: To express FNTB with a HA tag at C terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone size w/o insert (bp): 5137
• Total vector size (bp): 6448
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FNTB
• Alt name: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit beta
• Alt name: CAAX farnesyltransferase subunit beta
• Species: H. sapiens (human)
• Insert Size (bp): 1311
• Mutation: none
• GenBank ID: NM_002028.3.
• Entrez Gene: FNTB (a.k.a. FPTB)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry