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p{CFD4-3xP3::DsRed}
(Plasmid #86864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFD4-U6:1_U6:3tandemgRNAs
  • Backbone manufacturer
    Simon Bullock Lab
  • Backbone size (bp) 7159
  • Modifications to backbone
    Replaced vermillion gene with 3XP3::DsRed. Then mutated DsRed to eliminate BbsI restriction site
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer accgcgggcccgggatccaccggtcgccacCATGGTGCGCTCCTCCAA
  • 3′ sequencing primer gtacaggcgctcggtggaggcctcccagccCATGGTTTTCTTCTGCATTACGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p{CFD4-3xP3::DsRed} was a gift from David Stern (Addgene plasmid # 86864 ; http://n2t.net/addgene:86864 ; RRID:Addgene_86864)

  • For your References section:

    Genetic and Transgenic Reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis. Stern DL, Crocker J, Ding Y, Frankel N, Kappes G, Kim E, Kuzmickas R, Lemire A, Mast JD, Picard S. G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 10.1534/g3.116.038885. 10.1534/g3.116.038885 PubMed 28280212
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