pcDNA5/FRT/TO-FNTA-FLAG
(Plasmid #86858)

• Purpose: To express FNTA with a FLAG tag at C terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone size w/o insert (bp): 5137
• Total vector size (bp): 6067
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FNTA
• Alt name: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
• Alt name: CAAX farnesyltransferase subunit alpha
• Species: H. sapiens (human)
• Insert Size (bp): 936
• Mutation: The sequence of the insert is isoform 2, which differs from the canonical sequence as follows: 68-134: Missing.
• GenBank ID: NM_002027.2
• Entrez Gene: FNTA (a.k.a. FPTA, PGGT1A, PTAR2)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry