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Addgene

pBOB-EF1-FastFUCCI-Puro Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pBOB-EF1-FastFUCCI-Puro with the following enzymes: BamHI-HF, XhoI, and SpeI-HF.

Expected Bands
BamHI-HF: 12.4 kb
XhoI: 12.4 kb
SpeI-HF: 6.5 kb & 5.8 kb

We observe the expected bands for this digest.