HA-mEOS3.2-TRF2 HR Donor
(Plasmid
#86845)
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PurposeHR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBac
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-mEOS3.2-TRF2 at endogenous locus
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SpeciesH. sapiens (human)
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Entrez GeneTERF2 (a.k.a. TRBF2, TRF2)
- Promoter Endogenous TRF2 promoter
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Tag
/ Fusion Protein
- HA-mEOS3.2 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer MBP forward
- 3′ sequencing primer SV40 poly A rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation R98K was found in Gentamicin during Addgene's quality control process.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-mEOS3.2-TRF2 HR Donor was a gift from Thomas Cech (Addgene plasmid # 86845 ; http://n2t.net/addgene:86845 ; RRID:Addgene_86845) -
For your References section:
Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres. Schmidt JC, Zaug AJ, Cech TR. Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11. 10.1016/j.cell.2016.07.033 PubMed 27523609
