FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
(Plasmid #86843)

• Purpose: HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
• Depositing Lab: Thomas Cech
• Publication: Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11.

Backbone

• Vector backbone: pFastBac
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLAG-HaloTag-TERT Exon 1 and part of Exon 2
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Promoter: Endogenous TERT promoter
• Tag / Fusion Protein:
  • FLAG-HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13f
• 3′ sequencing primer: M13r

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry