-
Purposemammalian expression of GFP-p38beta
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP-C1
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namep38beta
-
Alt namemitogen-activated protein kinase 11
-
SpeciesH. sapiens (human)
-
Entrez GeneMAPK11 (a.k.a. P38B, P38BETA2, PRKM11, SAPK2, SAPK2B, p38-2, p38Beta)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GFP-p38beta was a gift from Rony Seger (Addgene plasmid # 86833 ; http://n2t.net/addgene:86833 ; RRID:Addgene_86833) -
For your References section:
Beta-like importins mediate the nuclear translocation of mitogen-activated protein kinases. Zehorai E, Seger R. Mol Cell Biol. 2014 Jan;34(2):259-70. doi: 10.1128/MCB.00799-13. Epub 2013 Nov 11. 10.1128/MCB.00799-13 PubMed 24216760
