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Addgene

pCDNA3.1 (-) + codon optimized Brn3a
(Plasmid #86829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1 (-)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brn3a
  • Alt name
    POU4F1
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU4F1 (a.k.a. ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The wild-type sequence of Brn3a was codon optimized to allow for gene synthesis of gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1 (-) + codon optimized Brn3a was a gift from Carl Novina (Addgene plasmid # 86829 ; http://n2t.net/addgene:86829 ; RRID:Addgene_86829)

  • For your References section:

    The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH, Novina CD. Cell Rep. 2016 May 31;15(9):2025-37. doi: 10.1016/j.celrep.2016.04.018. Epub 2016 May 19. 10.1016/j.celrep.2016.04.018 PubMed 27210747
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