HOXB13 (human) Myc-tag-pSP72
(Plasmid
#8682)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP72
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2462
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Vector typeBacterial Expression ; TNT in vitro
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB13
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Alt nameSDX1
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SpeciesH. sapiens (human)
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Insert Size (bp)1058
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Mutationnew NsiI site at ATG start
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Entrez GeneHOXB13 (a.k.a. HPC9, PSGD)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site EcoRI in TA Vector to HindIII (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB13 (human) Myc-tag-pSP72 was a gift from Corey Largman (Addgene plasmid # 8682 ; http://n2t.net/addgene:8682 ; RRID:Addgene_8682) -
For your References section:
Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless fetal wounds. Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C. J Invest Dermatol 1998 Jul;111(1):57-63. 10.1046/j.1523-1747.1998.00238.x PubMed 9665387
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/22/29/13d9058e-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)