pCDNA3.1(-) + SLNCR1 _462-699
(Plasmid
#86819)
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Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1 (-)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLINC00673
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Alt nameHI-LNC75
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Alt nameLUCAIR1
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SpeciesH. sapiens (human)
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Entrez GeneLINC00673 (a.k.a. ERRLR01, HI-LNC75, HILNC75, LUCAIR1, SLNCR, SLNCR1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1(-) + SLNCR1 _462-699 was a gift from Carl Novina (Addgene plasmid # 86819 ; http://n2t.net/addgene:86819 ; RRID:Addgene_86819) -
For your References section:
The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH, Novina CD. Cell Rep. 2016 May 31;15(9):2025-37. doi: 10.1016/j.celrep.2016.04.018. Epub 2016 May 19. 10.1016/j.celrep.2016.04.018 PubMed 27210747