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Addgene

pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)
(Plasmid #86818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1 (-)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LINC00673
  • Alt name
    HI-LNC75
  • Alt name
    LUCAIR1
  • Species
    H. sapiens (human)
  • Entrez Gene
    LINC00673 (a.k.a. ERRLR01, HI-LNC75, HILNC75, LUCAIR1, SLNCR, SLNCR1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence) was a gift from Carl Novina (Addgene plasmid # 86818 ; http://n2t.net/addgene:86818 ; RRID:Addgene_86818)

  • For your References section:

    The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH, Novina CD. Cell Rep. 2016 May 31;15(9):2025-37. doi: 10.1016/j.celrep.2016.04.018. Epub 2016 May 19. 10.1016/j.celrep.2016.04.018 PubMed 27210747
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