MSCV-(mcs) human CSF1 full length-IRES-Puro
(Plasmid #86797)

• Purpose: retroviral expression of CSF-1
• Depositing Labs: Martine Roussel, Charles Sherr
• Publication: Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Backbone

• Vector backbone: MSCV-IRES-Puro
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CSF-1
• Alt name: macrophage colony stimulating factor 1
• Species: H. sapiens (human)
• Entrez Gene: CSF1 (a.k.a. CSF-1, MCSF, PG-M-CSF)
• Tag / Fusion Protein:
  • IRES-Puro (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN5; pBABE5
• 3′ sequencing primer: pCDH-rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The mcs is from the pBabe vector that was inserted into the MSCV vector to provide more cloning sites, and was developed by Dr Den Besten. The human CSF-1 cDNAs were cloned by the Cetus Company.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length CSF-1 is secreted