pFLEX+mCyclin D1
(Plasmid #86793)

• Purpose: mammalian expression of cyclin D1
• Depositing Labs: Martine Roussel, Charles Sherr
• Publication: Matsushime et al Cell. 1991 May 17;65(4):701-13.

Backbone

• Vector backbone: pFLEX
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D1
• Alt name: ccnd1
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Entrez Gene: Ccnd1 (a.k.a. CycD1, Cyl-1, PRAD1, bcl-1, cD1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Alan Diehl; when in Drs Sherr and Roussel laboratories

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mouse cyclin D1 wild type was cloned into the EcoRI site of pFLEX vector. In frame EcoRI site of FLAG-Tag mouse cyclin D1 was engineered by PCR and confirmed by sequence analysis. Protein expression confirmed by immunofluorescence and immunoblotting.