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pBluescript-mouse cyclin D2
(Plasmid #86791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS II KS+
  • Backbone size w/o insert (bp) 1200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D2
  • Alt name
    ccnd2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ccnd2 (a.k.a. 2600016F06Rik, Vin-1, Vin1, cD2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Matsushime when in Drs Sherr and Roussel laboratories

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript-mouse cyclin D2 was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 86791 ; http://n2t.net/addgene:86791 ; RRID:Addgene_86791)

  • For your References section:

    Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle. Matsushime H, Roussel MF, Ashmun RA, Sherr CJ. Cell. 1991 May 17;65(4):701-13. 0092-8674(91)90101-4 [pii] PubMed 1827757
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