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Addgene

HOXC13(human)-pET
(Plasmid #8679)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXC13
  • Alt name
    HOX3G
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    995
  • Mutation
    makes protein by Coomassie blue staining
  • GenBank ID
    NM_017410
  • Entrez Gene
    HOXC13 (a.k.a. ECTD9, HOX3, HOX3G)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alexander Awgulewitsch

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tkatchenko et al Development 128: 1547-1558 (2001)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXC13(human)-pET was a gift from Corey Largman (Addgene plasmid # 8679 ; http://n2t.net/addgene:8679 ; RRID:Addgene_8679)

  • For your References section:

    HOXB13 homeodomain protein is cytoplasmic throughout fetal skin development. Komuves LG, Ma XK, Stelnicki E, Rozenfeld S, Oda Y, Largman C. Dev Dyn 2003 Jun;227(2):192-202. 10.1002/dvdy.10290 PubMed 12761847
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