Flag Rab12 T56N
(Plasmid
#86789)
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Purposemammalian expression of Rab12 T56N, constitutively GDP-bound mutant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab12
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SpeciesH. sapiens (human)
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MutationT56N, constitutively GDP-bound mutant
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Entrez GeneRAB12
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag Rab12 T56N was a gift from Peter McPherson (Addgene plasmid # 86789 ; http://n2t.net/addgene:86789 ; RRID:Addgene_86789) -
For your References section:
Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy. Xu J, Fotouhi M, McPherson PS. EMBO Rep. 2015 Jun;16(6):709-18. doi: 10.15252/embr.201440006. Epub 2015 Apr 29. 10.15252/embr.201440006 PubMed 25925668
