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Purpose(Empty Backbone) for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Modifications to backboneSkylan-NS cDNA was cloned into the BamHI/Not I sites of pEGFP-N1
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- Skylan-NS (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Skylan was generated by mutating H62L of mEos3.1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSkylan-NS-N1 was a gift from Pingyong Xu (Addgene plasmid # 86785 ; http://n2t.net/addgene:86785 ; RRID:Addgene_86785) -
For your References section:
Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy. Zhang X, Zhang M, Li D, He W, Peng J, Betzig E, Xu P. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10364-9. doi: 10.1073/pnas.1611038113. Epub 2016 Aug 25. 10.1073/pnas.1611038113 PubMed 27562163
