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Addgene

stathmin-CFP
(Plasmid #86783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    stathmin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    502
  • Entrez Gene
    STMN1 (a.k.a. C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN)
  • Promoter CMV
  • Tag / Fusion Protein
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    stathmin-CFP was a gift from Lynne Cassimeris (Addgene plasmid # 86783 ; http://n2t.net/addgene:86783 ; RRID:Addgene_86783)

  • For your References section:

    Stathmin regulates centrosomal nucleation of microtubules and tubulin dimer/polymer partitioning. Ringhoff DN, Cassimeris L. Mol Biol Cell. 2009 Aug;20(15):3451-8. doi: 10.1091/mbc.E09-02-0140. Epub 2009 Jun 10. 10.1091/mbc.e09-02-0140 PubMed 19515833
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