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PurposeMammalian expression of stathmin-GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namestathmin
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SpeciesH. sapiens (human)
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Insert Size (bp)502
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Entrez GeneSTMN1 (a.k.a. C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
stathmin-GFP was a gift from Lynne Cassimeris (Addgene plasmid # 86782 ; http://n2t.net/addgene:86782 ; RRID:Addgene_86782) -
For your References section:
Stathmin regulates centrosomal nucleation of microtubules and tubulin dimer/polymer partitioning. Ringhoff DN, Cassimeris L. Mol Biol Cell. 2009 Aug;20(15):3451-8. doi: 10.1091/mbc.E09-02-0140. Epub 2009 Jun 10. 10.1091/mbc.e09-02-0140 PubMed 19515833
