FKBP*-PTEN
(Plasmid #86781)

• Purpose: Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
• Depositing Lab: Britta Eickholt
• Publication: An et al PLoS One. 2015 Dec 30;10(12):e0145783. doi: 10.1371/journal.pone.0145783. eCollection 2015.

Backbone

• Vector backbone: pCAX
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PTEN
• Alt name: phosphatase and tensin homolog
• Species: H. sapiens (human)
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Tags / Fusion Proteins:
  • FKBP* (F36V and L106P) (N terminal on insert)
  • linker (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG

Resource Information

• Supplemental Documents:
  • ABE58_1p125HN81_pCAX_FKBPE31sD32s-PTEN (1).ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry