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Addgene

FtHAP-pNIC-Bsa4
(Plasmid #86778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-Bsa4
  • Backbone size w/o insert (bp) 5356
  • Total vector size (bp) 6358
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Histidine Acid Phosphatase
  • Species
    Francisella tularensis
  • Insert Size (bp)
    1002
  • Tag / Fusion Protein
    • 6x His tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TACTTCCAATCCATGGTGGGTTACTCTTCT
  • 3′ sequencing primer TATCCACCTTTACTGTTATTTCTGGAATTTTTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FtHAP-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 86778 ; http://n2t.net/addgene:86778 ; RRID:Addgene_86778)
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