pcsII-pb2-flag
(Plasmid
#86766)
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PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSII-EF2-MCS
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameproteasome beta subunit 2
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SpeciesH. sapiens (human)
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Insert Size (bp)606
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Entrez GenePSMB2 (a.k.a. HC7-I)
- Promoter EF-1a
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcsII-pb2-flag was a gift from Do-Hyung Kim (Addgene plasmid # 86766 ; http://n2t.net/addgene:86766 ; RRID:Addgene_86766) -
For your References section:
mTORC1 Coordinates Protein Synthesis and Immunoproteasome Formation via PRAS40 to Prevent Accumulation of Protein Stress. Yun YS, Kim KH, Tschida B, Sachs Z, Noble-Orcutt KE, Moriarity BS, Ai T, Ding R, Williams J, Chen L, Largaespada D, Kim DH. Mol Cell. 2016 Feb 18;61(4):625-39. doi: 10.1016/j.molcel.2016.01.013. Epub 2016 Feb 11. 10.1016/j.molcel.2016.01.013 PubMed 26876939