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Addgene

HA-PRAS40 AA
(Plasmid #86759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human PRAS40
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    771
  • Mutation
    AA (S183A, S221A)
  • Entrez Gene
    AKT1S1 (a.k.a. Lobe, PRAS40)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Image# 2988649

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-PRAS40 AA was a gift from Do-Hyung Kim (Addgene plasmid # 86759 ; http://n2t.net/addgene:86759 ; RRID:Addgene_86759)

  • For your References section:

    mTORC1 Coordinates Protein Synthesis and Immunoproteasome Formation via PRAS40 to Prevent Accumulation of Protein Stress. Yun YS, Kim KH, Tschida B, Sachs Z, Noble-Orcutt KE, Moriarity BS, Ai T, Ding R, Williams J, Chen L, Largaespada D, Kim DH. Mol Cell. 2016 Feb 18;61(4):625-39. doi: 10.1016/j.molcel.2016.01.013. Epub 2016 Feb 11. 10.1016/j.molcel.2016.01.013 PubMed 26876939
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