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Addgene

mCherry-DFCP1
(Plasmid #86746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human DFCP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2334
  • Entrez Gene
    ZFYVE1 (a.k.a. DFCP1, PPP1R172, SR3, TAFF1, ZNFN2A1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IMAGE:5493027

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-DFCP1 was a gift from Do-Hyung Kim (Addgene plasmid # 86746 ; http://n2t.net/addgene:86746 ; RRID:Addgene_86746)

  • For your References section:

    mTORC1 phosphorylates UVRAG to negatively regulate autophagosome and endosome maturation. Kim YM, Jung CH, Seo M, Kim EK, Park JM, Bae SS, Kim DH. Mol Cell. 2015 Jan 22;57(2):207-18. doi: 10.1016/j.molcel.2014.11.013. Epub 2014 Dec 18. 10.1016/j.molcel.2014.11.013 PubMed 25533187
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