EGFP-UVRAG S498A
(Plasmid
#86741)
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PurposeN-terminal EGFP-tagged protein expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman UVRAG
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SpeciesH. sapiens (human)
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Insert Size (bp)2100
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MutationS498A
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Entrez GeneUVRAG (a.k.a. DHTX, VPS38, p63)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IMAGE: 6186856
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-UVRAG S498A was a gift from Do-Hyung Kim (Addgene plasmid # 86741 ; http://n2t.net/addgene:86741 ; RRID:Addgene_86741) -
For your References section:
mTORC1 phosphorylates UVRAG to negatively regulate autophagosome and endosome maturation. Kim YM, Jung CH, Seo M, Kim EK, Park JM, Bae SS, Kim DH. Mol Cell. 2015 Jan 22;57(2):207-18. doi: 10.1016/j.molcel.2014.11.013. Epub 2014 Dec 18. 10.1016/j.molcel.2014.11.013 PubMed 25533187
