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Addgene

HA-UVRAG
(Plasmid #86738)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86738 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human UVRAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    UVRAG (a.k.a. DHTX, VPS38, p63)
  • Promoter CMV
  • Tag / Fusion Protein
    • JA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IMAGE: 6186853

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-UVRAG was a gift from Do-Hyung Kim (Addgene plasmid # 86738 ; http://n2t.net/addgene:86738 ; RRID:Addgene_86738)

  • For your References section:

    mTORC1 phosphorylates UVRAG to negatively regulate autophagosome and endosome maturation. Kim YM, Jung CH, Seo M, Kim EK, Park JM, Bae SS, Kim DH. Mol Cell. 2015 Jan 22;57(2):207-18. doi: 10.1016/j.molcel.2014.11.013. Epub 2014 Dec 18. 10.1016/j.molcel.2014.11.013 PubMed 25533187
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