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Addgene

pBN154
(Plasmid #86718)

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Item Catalog # Description Quantity Price (USD)
Plasmid 86718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBN8
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBN154 was a gift from Peter Askjaer (Addgene plasmid # 86718 ; http://n2t.net/addgene:86718 ; RRID:Addgene_86718)

  • For your References section:

    An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene Expression in Caenorhabditis elegans. Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, Askjaer P. Genetics. 2017 Aug;206(4):1763-1778. doi: 10.1534/genetics.117.201012. Epub 2017 Jun 23. 10.1534/genetics.117.201012 PubMed 28646043
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