pBN40
(Plasmid
#86715)
-
PurposeC. elegans injection marker
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 86715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepPD49.83
-
Vector typeWorm Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePlmn-1::gfp::his-58
-
SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBN40 was a gift from Peter Askjaer (Addgene plasmid # 86715 ; http://n2t.net/addgene:86715 ; RRID:Addgene_86715) -
For your References section:
Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans. Morales-Martinez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Mar 15;128(6):1090-6. doi: 10.1242/jcs.164202. Epub 2015 Feb 4. 10.1242/jcs.164202 PubMed 25653391