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Addgene

pBN1
(Plasmid #86710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJH4.52
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plmn-1::mCherry::his-58::pie-1 3'UTR
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBN1 was a gift from Peter Askjaer (Addgene plasmid # 86710 ; http://n2t.net/addgene:86710 ; RRID:Addgene_86710)

  • For your References section:

    Dissection of the NUP107 nuclear pore subcomplex reveals a novel interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis elegans. Rodenas E, Gonzalez-Aguilera C, Ayuso C, Askjaer P. Mol Biol Cell. 2012 Mar;23(5):930-44. doi: 10.1091/mbc.E11-11-0927. Epub 2012 Jan 11. 10.1091/mbc.E11-11-0927 PubMed 22238360
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