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Addgene

CROPseq-Guide-Puro Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid CROPseq-Guide-Puro with the following enzymes: BsmBI and SphI.

We observed the expected digest results-

BsmBI: 8.3kb & 1.9kb
SphI: 5.3kb, 3.8kb, 1.1kb