Skip to main content
Addgene

pRN3P-HA-Suv4-20h2mut
(Plasmid #86692)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRN3p
  • Backbone size w/o insert (bp) 3203
  • Total vector size (bp) 4610

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    histone-lysine N-methyltransferase KMT5C mutant
  • Alt name
    Suv4-20h2 mutant
  • Alt name
    KMT5C
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1421
  • Mutation
    Mutated asparagine 273 to cysteine 276 (NHDC) into AAAG (wild-type sequence CAACCATGACTG to mutated sequence CGCCGCTGCCGG)
  • GenBank ID
    AY555193.1
  • Entrez Gene
    Kmt5c (a.k.a. Suv4-20h2, Suv420h2)
  • Promoter T3
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xbai (not destroyed)
  • 3′ cloning site Noti (not destroyed)
  • 5′ sequencing primer T3 sequence
  • 3′ sequencing primer T7 sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN3P-HA-Suv4-20h2mut was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 86692 ; http://n2t.net/addgene:86692 ; RRID:Addgene_86692)

  • For your References section:

    SUV4-20 activity in the preimplantation mouse embryo controls timely replication. Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME. Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5. 10.1101/gad.288969.116 PubMed 27920088
Related items:
Commonly requested with: