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Addgene

pRB2065
(Plasmid #8669)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC119
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Don't know
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TUB1 upstream of LEU2
  • Alt name
    TUB1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TUB1 (a.k.a. YML085C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comments (unknown if destroyed)
  • 3′ cloning site See comments (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119.
Then, BamHI-SalI fragment from pJJ282 (LEU2 gene) Klenow filled and inserted into the HpaI site, downstream of TUB1; LEU2 is oriented in the same direction as TUB1. For information on pUC119, search for pUC119 in Addgene's vector db.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB2065 was a gift from David Botstein (Addgene plasmid # 8669 ; http://n2t.net/addgene:8669 ; RRID:Addgene_8669)

  • For your References section:

    Structure-function relationships in yeast tubulins. Richards KL, Anders KR, Nogales E, Schwartz K, Downing KH, Botstein D. Mol Biol Cell 2000 May;11(5):1887-903. 10.1091/mbc.11.5.1887 PubMed 10793159
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