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Addgene

pRN3P-HA-Suv4-20h1
(Plasmid #86689)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86689 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRN3p-HA
  • Backbone size w/o insert (bp) 3203
  • Total vector size (bp) 5840

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    histone-lysine N-methyltransferase KMT5B
  • Alt name
    KMT5B
  • Alt name
    Suv4-20h1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2663
  • GenBank ID
    AY555192.1
  • Entrez Gene
    Kmt5b (a.k.a. AA117471, C630029K18Rik, Suv4-20h1, Suv420h1)
  • Promoter T3
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bamhi (not destroyed)
  • 3′ cloning site Noti (not destroyed)
  • 5′ sequencing primer T3 sequence
  • 3′ sequencing primer T7 sequence
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN3P-HA-Suv4-20h1 was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 86689 ; http://n2t.net/addgene:86689 ; RRID:Addgene_86689)
  • For your References section:

    SUV4-20 activity in the preimplantation mouse embryo controls timely replication. Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME. Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5. 10.1101/gad.288969.116 PubMed 27920088