pRN3P-HA-Suv4-20h1
(Plasmid
#86689)
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PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRN3p-HA
- Backbone size w/o insert (bp) 3203
- Total vector size (bp) 5840
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehistone-lysine N-methyltransferase KMT5B
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Alt nameKMT5B
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Alt nameSuv4-20h1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2663
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GenBank IDAY555192.1
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Entrez GeneKmt5b (a.k.a. AA117471, C630029K18Rik, Suv4-20h1, Suv420h1)
- Promoter T3
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Tag
/ Fusion Protein
- HA-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bamhi (not destroyed)
- 3′ cloning site Noti (not destroyed)
- 5′ sequencing primer T3 sequence
- 3′ sequencing primer T7 sequence (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRN3P-HA-Suv4-20h1 was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 86689 ; http://n2t.net/addgene:86689 ; RRID:Addgene_86689) -
For your References section:
SUV4-20 activity in the preimplantation mouse embryo controls timely replication. Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME. Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5. 10.1101/gad.288969.116 PubMed 27920088