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pRB327
(Plasmid #8668)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YEp21
  • Backbone manufacturer
    Botstein et al. Gene. 1979 8(1):17-24.
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Don't know
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TUB1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TUB1 (a.k.a. YML085C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comments (unknown if destroyed)
  • 3′ cloning site See comments (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A TUB1 fragment was ligated from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI (??) fragment of YEp21. For more information on YEp21, search for YEp21 in Addgene's vector db.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB327 was a gift from David Botstein (Addgene plasmid # 8668 ; http://n2t.net/addgene:8668 ; RRID:Addgene_8668)

  • For your References section:

    Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Schatz PJ, Solomon F, Botstein D. Mol Cell Biol 1986 Nov;6(11):3722-33. PubMed 3540600
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