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Addgene

pRB326
(Plasmid #8667)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCp50
  • Backbone manufacturer
    Kuo et al, Mol Cell Biol. 1983 October; 3(10): 1730
  • Vector type
    Yeast Expression ; Centromeric
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Don't know
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TUB1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TUB1 (a.k.a. YML085C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comment (unknown if destroyed)
  • 3′ cloning site See comment (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The TUB1 centromere plasmid was constructed by ligating a TUB1 fragment from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI fragment of YCp50. For more information on YCp50, including map, search for YCp50 in Addgene's vector db.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB326 was a gift from David Botstein (Addgene plasmid # 8667 ; http://n2t.net/addgene:8667 ; RRID:Addgene_8667)

  • For your References section:

    Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Schatz PJ, Solomon F, Botstein D. Mol Cell Biol 1986 Nov;6(11):3722-33. PubMed 3540600
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