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Addgene

p3XFlag-Mettl4
(Plasmid #86665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-EYFP Addgene #34582
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mettl4
  • Alt name
    Methyltransferase-like 4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1350
  • Entrez Gene
    Mettl4 (a.k.a. 2410198H06Rik, A730091E08Rik, AV296509, HsT661)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EYFP not expressed due to stop codon in Mettl4.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFlag-Mettl4 was a gift from Douglas Feldman (Addgene plasmid # 86665 ; http://n2t.net/addgene:86665 ; RRID:Addgene_86665)

  • For your References section:

    An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman DE. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018. 10.1016/j.molcel.2019.03.018 PubMed 30982744
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