pRB306
(Plasmid
#8666)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Backbone manufacturerBolivar et al., Gene. 1977;2(2):95-113.
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Growth instructionsDon't know
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTUB1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneTUB1 (a.k.a. YML085C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information on pBR322, including a map, search for pBR322 in Addgene's vector db.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB306 was a gift from David Botstein (Addgene plasmid # 8666 ; http://n2t.net/addgene:8666 ; RRID:Addgene_8666) -
For your References section:
Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Schatz PJ, Solomon F, Botstein D. Mol Cell Biol 1986 Nov;6(11):3722-33. PubMed 3540600