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CDk4-luciferase fragment B
(Plasmid #86659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK4 promoter
  • Species
    H. sapiens (human)
  • Mutation
    generated by cleavage at the KpnI site from 880 (-1 to -422)
  • Entrez Gene
    CDK4 (a.k.a. CMM3, PSK-J3)
  • Promoter CDK7
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found some nucleotide discrepancies compared to reference sequence AY034380; however depositing lab confirms plasmid functionality

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CDk4-luciferase fragment B was a gift from Jaime Modiano (Addgene plasmid # 86659 ; http://n2t.net/addgene:86659 ; RRID:Addgene_86659)

  • For your References section:

    NFATc2-mediated repression of cyclin-dependent kinase 4 expression. Baksh S, Widlund HR, Frazer-Abel AA, Du J, Fosmire S, Fisher DE, DeCaprio JA, Modiano JF, Burakoff SJ. Mol Cell. 2002 Nov;10(5):1071-81. S1097276502007013 [pii] PubMed 12453415
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