CDK4-luciferase active (880)
(Plasmid
#86657)
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PurposeLuciferase reporter containing CDK4 promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDK4 promoter
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SpeciesH. sapiens (human)
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Mutation867 base pairs including up to coding nucleotide at position +43
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Entrez GeneCDK4 (a.k.a. CMM3, PSK-J3)
- Promoter CDK5
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS found some nucleotide discrepancies compared to reference sequence AY034380; however depositing lab confirms plasmid functionality
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CDK4-luciferase active (880) was a gift from Jaime Modiano (Addgene plasmid # 86657 ; http://n2t.net/addgene:86657 ; RRID:Addgene_86657) -
For your References section:
NFATc2-mediated repression of cyclin-dependent kinase 4 expression. Baksh S, Widlund HR, Frazer-Abel AA, Du J, Fosmire S, Fisher DE, DeCaprio JA, Modiano JF, Burakoff SJ. Mol Cell. 2002 Nov;10(5):1071-81. S1097276502007013 [pii] PubMed 12453415
