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Addgene

alpha4-nAChR shRNA (Alpha-4.3)
(Plasmid #86652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHRNA4 (alpha4-nAChR)
  • gRNA/shRNA sequence
    ATGAGAAGAACCAGATGAATGA
  • Species
    H. sapiens (human)
  • Entrez Gene
    CHRNA4 (a.k.a. BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4)
  • Promoter H1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found that the "A" nucleotide at position 19 within the shRNA sequence is missing; depositing lab feels that functionality is likely maintained

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha4-nAChR shRNA (Alpha-4.3) was a gift from Jaime Modiano (Addgene plasmid # 86652 ; http://n2t.net/addgene:86652 ; RRID:Addgene_86652)
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