p4459 FLAG-hPLIC-1 NTF
(Plasmid #8664)

• Depositing Lab: Peter Howley
• Publication: Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hPLIC-1
• Alt name: PLIC-1
• Species: H. sapiens (human)
• Mutation: N-terminal fragment: amino acids 1-112 of hPLIC-1 fused to peptide CYRFSRVYRH
• Entrez Gene: UBQLN1 (a.k.a. DA41, DSK2, PLIC-1, UBQN, XDRP1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map.