p4456 FLAG-hPLIC-2 NTF
(Plasmid
#8662)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV4
- Backbone size w/o insert (bp) 4874
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehPLIC-2
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SpeciesH. sapiens (human)
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MutationN-terminal fragment: amino acids 1-107 of hPLIC-2
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Entrez GeneUBQLN2 (a.k.a. ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4456 FLAG-hPLIC-2 NTF was a gift from Peter Howley (Addgene plasmid # 8662 ; http://n2t.net/addgene:8662 ; RRID:Addgene_8662) -
For your References section:
The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome. Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill G, Howley PM. Mol Cell 2000 Aug;6(2):409-19. 10.1016/S1097-2765(00)00040-X PubMed 10983987
Map uploaded by the depositor.
