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Addgene

p4455 FLAG-hPLIC-2
(Plasmid #8661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPLIC-2
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBQLN2 (a.k.a. ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4455 FLAG-hPLIC-2 was a gift from Peter Howley (Addgene plasmid # 8661 ; http://n2t.net/addgene:8661 ; RRID:Addgene_8661)

  • For your References section:

    The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome. Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill G, Howley PM. Mol Cell 2000 Aug;6(2):409-19. 10.1016/S1097-2765(00)00040-X PubMed 10983987
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