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Addgene

pRAF-MutL(R95F)
(Plasmid #86608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli MutL(R95F)
  • Species
    E. coli
  • Insert Size (bp)
    1898
  • Mutation
    R95F
  • GenBank ID
    NP_418591 NP_418591
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag and ald-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAF-MutL(R95F) was a gift from Richard Fishel (Addgene plasmid # 86608 ; http://n2t.net/addgene:86608 ; RRID:Addgene_86608)

  • For your References section:

    Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair. Liu J, Hanne J, Britton BM, Bennett J, Kim D, Lee JB, Fishel R. Nature. 2016 Nov 16;539(7630):583-587. doi: 10.1038/nature20562. 10.1038/nature20562 PubMed 27851738
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