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Addgene

p4580 AU-IRF-3a
(Plasmid #8660)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRF-3a
  • Alt name
    IRF-3
  • Species
    H. sapiens (human)
  • Entrez Gene
    IRF3 (a.k.a. IIAE7)
  • Tag / Fusion Protein
    • AU (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4580 AU-IRF-3a was a gift from Peter Howley (Addgene plasmid # 8660 ; http://n2t.net/addgene:8660 ; RRID:Addgene_8660)

  • For your References section:

    Functional characterization of interferon regulatory factor 3a (IRF-3a), an alternative splice isoform of IRF-3. Karpova AY, Ronco LV, Howley PM. Mol Cell Biol 2001 Jul;21(13):4169-76. 10.1128/MCB.21.13.4169-4176.2001 PubMed 11390646
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