MBP-TEV-VMA Model vector
(Plasmid #86590)

• Purpose: This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI
• Depositing Lab: Sharon Rozovsky
• Publication: Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.

Backbone

• Vector backbone: pMAL-c5X
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: VMA intein
• Species: S. cerevisiae (budding yeast)
• Mutation: Original SapI site from pMal was mutated out
• Promoter: Tac
• Tag / Fusion Protein:
  • His6-MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: MBP-F
• 3′ sequencing primer: TGTCCTACTCAGGAGAGCGTTCAC, ACCCATGACCTTATTACCAACCTC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

TGTCCTACTCAGGAGAGCGTTCAC to sequence VMA C-term; and ACCCATGACCTTATTACCAACCTC to sequence the insert between MBP C-term and VMA