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Addgene

Ubiquitin K48C/G76C
(Plasmid #86585)

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Item Catalog # Description Quantity Price (USD)
Plasmid 86585 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMAL-c5X
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Species
    H. sapiens (human)
  • Mutation
    TEV site; K48C/G76C
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter Tac
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubiquitin K48C/G76C was a gift from Sharon Rozovsky (Addgene plasmid # 86585 ; http://n2t.net/addgene:86585 ; RRID:Addgene_86585)

  • For your References section:

    Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations. Liu J, Chen Q, Rozovsky S. J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991. 10.1021/jacs.6b10991 PubMed 28186733
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